Dual gene‐activated dermal scaffolds regulate angiogenesis and wound healing by mediating the coexpression of <scp>VEGF</scp> and angiopoietin‐1

The vascularization of dermal substitutes is a key challenge in efforts to heal deep skin defects. In this study, dual gene-activated scaffolds (DGADSs-1) were fabricated by loading nanocomposite particles polyethylenimine (PEI)/multiple plasmid DNAs (pDNAs) encoding vascular endothelial growth factor and angiopoietin-1 at ratio 1:1. similar manner, DGADSs-2 loaded with chimeric both VEGF Ang-1. vitro studies showed that types DGADSs released PEI/pDNA nanoparticles sustained manner; they demonstrated effective transfection ability, leading upregulated expression Furthermore, promoted fibroblast proliferation blood vessel formation, although DGADSs-1 more obvious promotion effect. A rat full-thickness defect model split-thickness transplanted using one-step method could achieve full survival the 12th day after surgery groups, time was significantly shortened. Compared other three groups scaffolds, group had greater cell infiltration, collagen deposition, neovascularization, maturation, all which wound healing. Thus, compared single-gene-activated show potential for enhancing angiogenesis. different modes also exhibited differences terms angiogenesis; effect two genes better than gene (DGADSs-2). summary, DGADSs, continuously upregulate Ang-1 expression, offer new functional tissue-engineered substitute ability activate vascularization.